Dynamic Homeostasis

The classical purview deems the mitochondrion or cell membrane as a proton-impervious and highly selective/closed barrier. This does not explain how cells can dynamically redox homeostasize or how certain products formed in high throughput reactions (like mitochondrial oxidative phosphorylation) can spontaneously maintain osmotic balance.

In the chemiosmosis purview, ATP breakdown and synthesis occurs by the same reaction, ATP + Water = ADP + Pi. There is no chemical connectivity to the oxidation of NADH. In the murburn perspective, ATP breaks down with the classical reaction, but is synthesized in a directly coupled chemical reaction: ADP+ Pi + NADH + O₂ + H⁺ = ATP + NAD⁺ + H₂O + H₂O₂

In this bimolecular reaction scheme, while there are 5 reactants (and involve the intermediacy of multiple DROS), products are 3 (excluding water, the solvent). As a result, water moves out spontaneously due to colligative properties.

The membrane in the murburn purview permits proton entry (like any other phospholipid membrane), to balance the excess negative charges (resulting due to effective charge separation aided by the redox-active membrane proteins and due to the mobility and reactivity of oxygen) on the internal side of the membrane.