Publications & Presentations

Authors: Kelath Murali Manoj*, Vidhu Soman, Vivian David Jacob, Abhinav Parashar, Daniel Andrew Gideon, Manish Kumar, Afsal Manekkathodi, Surjith Ramasamy, Kannan Pakshirajan, Nikolai Mikhailovich Bazhin

Publication date: 2019/11/15

Journal: Archives of Biochemistry and Biophysics 376, 108128. (Invited review)

DOI: 10.1016/j.abb.2019.108128

33. Are adipocytes and ROS villains, or are they protagonists in the drama of life? The murburn perspective

Authors: Vivian David Jacob, Kelath Murali Manoj*

Publication date: 2019/9/3

Journal: Adipobiology 10, 7-16. (Cover-page credits)

DOI: 10.14748/adipo.v10.6534

32. Torday’s prognosis for aging and mortality: more evolution and better life!

Authors: Kelath Murali Manoj*

Publication date: 2019/5/1

Journal: Biomedical Reviews 30, 23-24

DOI: 10.14748/bmr.v30.6384

31. 2020: murburn concept heralds a new era in cellular bioenergetics

Authors: Daniel Andrew Gideon, Vivian David Jacob, Kelath Murali Manoj*

Publication date: 2019/5/1

Journal: Biomedical Reviews 30, 89-98. (Cover-page credits)

DOI: 10.14748/bmr.v30.6390

30. The ubiquitous biochemical logic of murburn concept

Authors: Kelath Murali Manoj*

Publication date: 2018/12/15

Journal: Biomedical Reviews 29, 89-97.

DOI: 10.14748/bmr.v29.5854

29. Murburn scheme for mitochondrial thermogenesis

Authors: Kelath Murali Manoj*, Daniel Andrew Gideon, Vivian David Jacob

Publication date: 2018/12/15

Journal: Biomedical Reviews 29, 73-82. (Cover-page credits)

DOI: 10.14748/bmr.v29.5852

28. Aerobic respiration: Criticism of the proton-centric explanation involving rotary adenosine triphosphate synthesis, chemiosmosis principle, proton pumps and electron transport chain

Authors: Kelath Murali Manoj*

Publication date: 2018/12

Journal: Biochemistry Insights 11, 1178626418818442

DOI: 10.1177/1178626418818442

27. Murburn concept: A molecular explanation for hormetic and idiosyncratic dose responses

Authors: Abhinav Parashar, Daniel Andrew Gideon, Kelath Murali Manoj*

Publication date: 2018/5/9

Journal: Dose-Response 16, 1559325818774421

DOI: 10.1177/1559325818774421

26. Debunking chemiosmosis and proposing murburn concept as the operative principle for cellular respiration

Authors: Kelath Murali Manoj*

Publication date: 2018/3/22

Journal: Biomedical Reviews 28, 31-48. (Cover-page credits)

DOI: 10.14748/bmr.v28.4450

25. Functioning of drug-metabolizing microsomal cytochrome P450s: In silico probing of proteins suggests that the distal heme ‘active site’pocket plays a relatively ‘passive role …

Authors: Avanthika Venkatachalam, Abhinav Parashar, Kelath Murali Manoj*

Publication date: 2016/12

Journal: In silico Pharmacology 4, 1-38.

DOI: 10.1186/s40203-016-0016-7

24. Functioning of microsomal cytochrome P450s: Murburn concept explains the metabolism of xenobiotics in hepatocytes

Authors: Kelath Murali Manoj*, Abhinav Parashar, Sudeep K Gade, Avanthika Venkatachalam

Publication date: 2016/6/23

Journal: Frontiers in pharmacology 7, 161.

DOI: 10.3389/fphar.2016.00161

23. Atypical profiles and modulations of heme-enzymes catalyzed outcomes by low amounts of diverse additives suggest diffusible radicals' obligatory involvement in such redox reactions

Authors: Kelath Murali Manoj*, Abhinav Parashar, Avanthika Venkatachalam, Sahil Goyal, Preeti Gunjan Singh, Sudeep K Gade, Kalaiselvi Periyasami, Reeba Susan Jacob, Debosmita Sardar, Shanikant Singh, Rajan Kumar, Daniel A Gideon

Publication date: 2016/6/1

Journal: Biochimie 125, 91-111.

DOI: 10.1016/j.biochi.2016.03.003

22. Metabolism of xenobiotics by cytochrome P450: novel insights into the thermodynamics, kinetics and roles of redox proteins and diffusible reactive species

Authors: Kelath Murali Manoj*, Avanthika Venkatachalam, Abhinav Parashar

Publication date: 2016/1/1

Conference: Drug Metabolism Reviews 48, 41-42.

DOI: 10.1080/03602532.2016.1191843

21. Electron transfer amongst flavo-and hemo-proteins: diffusible species effect the relay processes, not protein–protein binding

Authors: Kelath Murali Manoj*, Sudeep K Gade, Avanthika Venkatachalam, Daniel A Gideon

Publication date: 2016/2/23

Journal: RSC Advances 6, 24121-24129.

DOI: 10.1039/C5RA26122H

20. Cyanide does more to inhibit heme enzymes, than merely serving as an active-site ligand

Authors: Abhinav Parashar, Avanthika Venkatachalam, Daniel Andrew Gideon, Kelath Murali Manoj*

Publication date: 2014/12/12

Journal: Biochemical and Biophysical Research Communications 455, 190-193.

DOI: 10.1016/j.bbrc.2014.10.137

19. The curious case of benzbromarone: insight into super-inhibition of cytochrome P450

Authors: Abhinav Parashar, Sudeep Kumar Gade, Mahesh Potnuru, Nandita Madhavan, Kelath Murali Manoj*

Publication date: 2014/3/3

Journal: PloS One 9, e89967

DOI: 10.1371/journal.pone.0089967

18. What is the functional role of N-terminal transmembrane helices in the metabolism mediated by liver microsomal cytochrome P450 and its reductase?

Authors: Daniel Andrew Gideon, Rashmi Kumari, Andrew M Lynn, Kelath Murali Manoj*

Publication date: 2012/5

Journal: Cell Biochemistry and Biophysics 63, 35-45.

DOI: 10.1007/s12013-012-9339-0

17. Redox active molecules cytochrome c and vitamin C enhance heme-enzyme peroxidations by serving as non-specific agents for redox relay

Authors: Sudeep Kumar Gade, Subarna Bhattacharya, Kelath Murali Manoj*

Publication date: 2012/3/9

Journal: Biochemical and Biophysical Research Communications 419, 211-214.

DOI: 10.1016/j.bbrc.2012.01.149

16. Traces of certain drug molecules can enhance heme-enzyme catalytic outcomes

Authors: Abhinav Parashar, Kelath Murali Manoj*

Publication date: 2012/1/20

Journal: Biochemical and Biophysical Research Communications 417, 101-1045.

DOI: 10.1016/j.bbrc.2011.12.090

15. The intriguing enhancement of chloroperoxidase mediated one-electron oxidations by azide, a known active-site ligand

Authors: Daniel Andrew, Lowell Hager, Kelath Murali Manoj*

Publication date: 2011/12/2

Journal: Biochemical and Biophysical Research Communications 415, 646-649.

DOI: 10.1016/j.bbrc.2011.10.128

14. Cytochrome P450 reductase: a harbinger of diffusible reduced oxygen species

Authors: Kelath Murali Manoj*, Sudeep Kumar Gade, Lazar Mathew

Publication date: 2010/10/13

Journal: PloS One 5, e13272.

DOI: 10.1371/journal.pone.0013272

13. Explaining the atypical reaction profiles of heme enzymes with a novel mechanistic hypothesis and kinetic treatment

Authors: Kelath Murali Manoj*, Arun Baburaj, Binoy Ephraim, Febin Pappachan, Pravitha Parapurathu Maviliparambathu, Umesh K Vijayan, Sivaprasad Valiyaveettil Narayanan, Kalaiselvi Periasamy, Ebi Ashley George, Lazar T Mathew

Publication date: 2010/5/17

Journal: PloS One 5, e10601

DOI: 10.1371/journal.pone.0010601

12. Chloroperoxidase, a janus enzyme

Authors: Kelath Murali Manoj*, Lowell P Hager

Publication date: 2008/3/4

Journal: Biochemistry 47, 2997-3003

DOI: 10.1021/bi7022656

11. Chlorinations catalyzed by chloroperoxidase occur via diffusible intermediate (s) and the reaction components play multiple roles in the overall process

Authors: Kelath Murali Manoj*

Publication date: 2006/8/31

Journal: Biochimica et Biophysica Acta (BBA)-Proteins & Proteomics 1764, 1325-1339.

DOI: 10.1016/j.bbapap.2006.05.012

10. A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases

Authors: Kelath Murali Manoj*, Lowell P Hager

Publication date: 2006/1/1

Journal: Analytical Biochemistry 348, 84-86.

DOI: 10.1016/j.ab.2005.10.014

9. Two-dimensional NMR study of the heme active site structure of chloroperoxidase

Authors: Xiaotang Wang, Hiroyasu Tachikawa, Xianwen Yi, Kelath Murali Manoj, Lowell P Hager

Publication date: 2003/3/7

Journal: Journal of Biological Chemistry 278, 7765-7774.

DOI: 10.1074/jbc.M209462200

8. A spectrophotometric method to assay epoxide hydrolase activity

Authors: Tej Bhatnagar, Kelath Murali Manoj, Jacques C Baratti

Publication date: 2001/12/4

Journal: Journal of Biochemical and Biophysical Methods 50, 1-13.

DOI: 10.1016/S0165-022X(01)00162-2

7. Utilization of peroxide and its relevance in oxygen insertion reactions catalyzed by chloroperoxidase

Authors: Kelath Murali Manoj, Lowell P Hager

Publication date: 2001/6/11

Journal:Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1547, 408-417.

DOI: 10.1016/S0167-4838(01)00210-2

6. Microbiological transformations. Part 45: A green chemistry preparative scale synthesis of enantiopure building blocks of Eliprodil: elaboration of a high substrate concentration epoxide hydrolase-catalyzed hydrolytic kinetic resolution process

Authors: Kelath Murali Manoj, Alain Archelas, Jacques Baratti, Roland Furstoss

Publication date: 2001/1/21

Journal: Tetrahedron 57, 695-701.

DOI: 10.1016/S0040-4020(00)01032-2

5. Epoxidation of indene by chloroperoxidase

Authors: Kelath Murali Manoj, Frederick J Lakner, Lowell P Hager

Publication date: 2000/3/20

Journal: Journal of Molecular Catalysis B: Enzymatic 9, 107-111.

DOI: 10.1016/S1381-1177(99)00118-6

4. A kinetic epoxidation assay for chloroperoxidase

Authors: Kelath Murali Manoj, Xianwen Yi, Gyan P Rai, Lowell P Hager

Publication date: 1999/12/20

Journal: Biochemical and Biophysical Research Communications 266, 301-303.

DOI: 10.1006/bbrc.1999.1810

3. Replacement of the proximal heme thiolate ligand in chloroperoxidase with a histidine residue

Authors: Xianwen Yi, Mark Mroczko, Kelath Murali Manoj, Xiaotang Wang, Lowell P Hager

Publication date: 1999/10/26

Journal: Proceedings of the National Academy of Sciences 96, 12412-12417.

DOI: 10.1073/pnas.96.22.12412

2. Ester hydrolyses in reversed micelles using lipase

Authors: Kelath Murali Manoj, T Swaminathan

Publication date: 1997/8

Journal: Bioprocess Engineering 17, 185-188.

DOI: 10.1007/s004490050373

1. Physicochemical studies on reverse micelles of sodium bis (2-ethylhexyl) sulfosuccinate at low water content

Authors: Kelath Murali Manoj, R Jayakumar, SK Rakshit

Publication date: 1996/8/21

Journal: Langmuir 12, 4068-4072.

DOI: 10.1021/la950279a

National Outreach

2. KM Manoj* & Afsal Manekkathodi (2023). Murburn concept, a stochastic principle for evolution and synthesis of cellular functions. Proceedings of the 30th Swadeshi Science Congress. Cover-page, 69-82.

1. KM Manoj* (2023). Why is oxygen needed for life? Discrediting prevailing ideas, murburn concept affords viable explanations. Sasthra, The Indian Journal of Science and Technology 4 (6): 33-40.

Conference/Workshop Presentations on Murburn Concept

22. Kelath Murali Manoj* (December, 2023). Why the Z-scheme is redundant and how murburn model better explains photosynthesis. Keynote Lecture (10 minutes.) in Agriculture Session. 6th Bharatiya Vigyan Sammelan, Ahmedabad.

21. Kelath Murali Manoj* (December, 2023). Murburn concept, a paradigm shift in bioenergetics. Invited lecture (1 hour) in Emerging Science & Technology Session. 6th Bharatiya Vigyan Sammelan, Ahmedabad.

20. Kelath Murali Manoj* (May, 2023). Murzymes, murburn concept & oxygen: Explaining molecular to macroscopic phenomena in drug metabolism, mitochondrial respiration-thermogenesis, photoreception in retina and cellular electrophysiology. Presentation (25 minutes) at 30th Swadeshi Science Congress, NIT Calicut.

https://www.youtube.com/watch?v=UM8nSvbpdBg&t=1315s

19. Kelath Murali Manoj* (May, 2023). Murburn Concept: A stochastic principle for evolution & synthesis of cellular functions. Brief talk (10 minutes) at 30th Swadeshi Science Congress, NIT Calicut.

https://www.youtube.com/watch?v=rQBRzT4H-ic&t=585s

18. Kelath Murali Manoj* (May, 2023). Untenable Z-scheme & Kok-Joliot cycle: Murburn model of light reaction of photosynthesis. Brief talk (15 minutes) at 30th Swadeshi Science Congress, NIT Calicut.

https://www.youtube.com/watch?v=du199iQVT1Y&t=870s

17. Kelath Murali Manoj* (15-16 March, 2023). 2day Workshop on murburn concept. IIT Bombay, India; [~550 registrations from diverse parts of India and the world; hosted by Prof. Amit Sethi (EE), Prof. Sharad Bhartiya (ChE) & Prof. KV Venkatesh (ChE)]

https://www.youtube.com/watch?v=HX7f4II6q2M (Inauguration)
https://www.youtube.com/watch?v=LYZtuNhP918 (Session 1A: Origins of murburn concept, Catalysis by chloroperoxidase)
https://www.youtube.com/watch?v=qdjxBvktsSo (Session 1B: Drug/Xenobiotic metabolism / Biological Electron Transfers)
https://www.youtube.com/watch?v=Br2D8gb83gI (Session 2A: Respiration & Thermogenesis)
https://www.youtube.com/watch?v=18PCAQhOqNc (Session 2B: Oxygenic Photosynthesis)
https://www.youtube.com/watch?v=BTDAOYa84u8&t=122s (Session 3A: Phototransduction/Vision & Neuronal signal relay)
https://www.youtube.com/watch?v=r6aJ4jOAXLg (Session 3B: Electrophysiology & Erythrocytes)
https://www.youtube.com/watch?v=GUrLjasyyw8 (Session 4A: Lactate dehydrogenase, PTMs and Recapitulations)
https://www.youtube.com/watch?v=lTlR3U8_OKg (Session 4B: Bacterial flagella, Origin/Evolution of life, Overviews)
https://www.youtube.com/watch?v=d5cimYt1dds (All of the eight talks above, slides only)

16. Kelath Murali Manoj* (October, 2022). Cyclooxygenase, Complex V, Rhodopsin-Transducin and Na,K-ATPase are murzymes! Poster at 42nd Midwest Enzyme Chemistry Conference, Chicago, USA.

15. Kelath Murali Manoj* (July, 2022). How do we see? Why do we breathe? Keynote presentation (1 hour) at BIOVERVE, St. Joseph’s College, Iringalakkuda.

14. Kelath Murali Manoj* (March, 2022). Why do we use oxygen? How does small amounts of cyanide kill so quickly? Plenary Lecture (1 hour) at Frontiers in Chemical Sciences (FCS-2022) Department of Chemistry, University of Calicut.

13. Daniel Andrew Gideon*, Vijay Nirusimhan, Abhinav Parashar, Jesucastin Edward, Kelath Murali Manoj (October 2021). Murburn concept explicates the structure-function correlations of lactate dehydrogenase: Relevance in Cori cycle and Warburg effect. Poster at 41st Midwest Enzyme Chemistry Conference, Chicago, USA.

https://www.youtube.com/watch?v=YlJjpX1l-BI

12. Kelath Murali Manoj*, Abhinav Parashar, Daniel Andrew Gideon, Afsal Manekkathodi (October 2020). Respiratory/photosynthetic membrane protein complexes are murzymes that catalyze reactions by enhancing the interaction of diffusible species with ‘substrates’. Invited presentation at 40th Midwest Enzyme Chemistry Conference, Chicago, USA.

https://www.youtube.com/watch?v=O4AzYJ5S26Q&t=5s

11. Kelath Murali Manoj*, (January 2020) Plenary/Inaugural speech (1.5 hours) “Why do we need oxygen? How does cyanide kill so quickly?” at International Conference on Prospects of Biosciences in Transdisciplinary Research – 2020, BHC, Trichy, India.

10. Kelath Murali Manoj*, A Parashar, Avanthika V & D A Gideon (February 2016). Metabolism of xenobiotics mediated in liver microsomes by the enzymatic couplet of hemoprotein cytochrome P450 and flavoprotein reductase: “Radical” mechanistic insights reorient several decades’ paradigm. Poster at6th International Conference on Metals in Genetics and Biology, IISc Bangalore, India.

9. Kelath Murali Manoj*, Avanthika V & A Parashar (October 2015). Metabolism of xenobiotics by Cytochrome P450: Novel insights into the thermodynamics, kinetics and roles of redox proteins and diffusible species. Poster at 20th North American Meeting of ISSX, Orlando, USA.

8. Kelath Murali Manoj*, A Parashar & Avanthika V (September 2015). Going beyond Fisher/Koshland mechanisms and Michaelis-Menten kinetics: A new paradigm better explains Cytochrome P450 mediated drug metabolism. Poster at 35th Midwest Enzyme Chemistry Conference, Chicago, USA.

7. Kelath Murali Manoj*, Avanthika V, A Parashar & D A Gideon (July 2015). Challenging the prevailing understanding of CYP reaction mechanism: Obligatory requirement of diffusible species and operation by 'thermodynamic pull'. Poster at 45th Gordon Research Conference on Drug Metabolism, Holderness, USA.

6. Kelath Murali Manoj*, S K Gade, A Parashar, Avanthika V & D A Gideon (February 2015). Redox relay in Cytochrome P450 reactions: Involvement of diffusible species and 'thermodynamic pull' hypothesis. Poster at 7th International Symposium on Drug Metabolism and Pharmaco Kinetics (ISSX-NIPER), Mohali, India.

5. Kelath Murali Manoj* (June 2009). A hypothesis involving diffusible radical(s) explains some of the reactions catalyzed by cytochrome P450s. Poster at 16th International conference on Cytochrome P450s, Okinawa, Japan.

4. Sivaprasad V. N., Arun Baburaj, Binoy Ephraim, Febin Pappachan, Pravida P. M., Umesh K. Vijayan & Kelath Murali Manoj* (June 2009). Kinetics of one-electron oxidations by chloroperoxidase: What is the analogous take-home lesson for CYPs? Presentation at 16th International Conference on Cytochrome P450s, Okinawa, Japan.

3. Kelath Murali Manoj* (June 2007). A novel scheme to explain the mechanistic chemistry of CYP reactions. Poster at 15th International Conference on Cytochrome P450s, Bled, Slovenia.

2. Kelath Murali Manoj* & Lowell P. Hager (June 2007). Chloroperoxidase, a Janus enzyme. Presentation at 15th International Conference on Cytochrome P450s, Bled, Slovenia.

1. Kelath Murali Manoj* & Lowell P. Hager (June 2005). New insight into chlorinations catalyzed by chloroperoxidase. 14th International Conference on Cytochrome P450s at Dallas, Texas.

Presentations on Murburn Concept at Indian Institutions

15. St. Mary’s College, Thrissur. Biom-23: Three hours Interactive Presentation on murburn concept (Oct 4, 2023).

14. NIIST Trivandrum, 20th February, 2023; “The roles of membrane-embedded murzymes and oxygen in drug metabolism and respiration” (Or “Why murburn concept is the master of ceremonies in cellular functioning”)

13. RGCB Trivandrum, 10th June, 2022; “Murburn concept: A paradigm-shift idea in biology and medicine”

https://www.youtube.com/watch?v=LeNB721Cs00 (First half, part 1)
https://www.youtube.com/@satyamjayatu5613/videos (Second half, only a bit of part 2)
https://www.youtube.com/watch?v=nKFhYNMUJy4 (slides only)

12. IIT Palakkad, 5th March, 2020: “Why do we need oxygen? How does cyanide kill so quickly?”

11. IIT Madras, 18th March, 2019: “Approaching mitochondrial bioenergetics from the murburn perspective”

10. IISER Kolkata, 14th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

9. IACS Kolkata, 13th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

8. IASST Guwahati, 11th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

7. NEHU Shillong, 8th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

6. IIT Delhi, 4th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

5. JNU New Delhi, 4th March, 2019: “Why is oxygen the elixir of life? How does cyanide kill so quickly?”

4. CUSAT Cochin, 17th January, 2018: “Why do we need oxygen?”

3. IISc Bangalore, 19th May, 2017: “A radical answer to the question- Why do we need oxygen?”

2. IISER Trivandrum, 21st April, 2017: “Why/How do we use oxygen?”

1. IIT Madras, 17th April, 2017: “Why/How do we use oxygen?”